Glyphosate-resistant hairy fleabane documented in the Central Valley
In recent years, growers and pest consultants have reported poor control of the weed hairy fleabane in some areas of the Central Valley. Hairy fleabane seeds were collected from Esparto, Fresno and Reedley, Calif., and greenhouse-grown seedlings were treated at several different glyphosate rates and compared with an untreated control. None of the Esparto or Fresno plants survived glyphosate rates greater than 0.78 pounds acid equivalent per acre (lb ae/ac), while some of the plants from Reedley survived even the highest rate of glyphosate tested (12.4 lb ae/ac). The dose required to reduce plant dry weights by 50% (GR50) of the Esparto plants ranged from 0.28 to 0.30 lb ae/ac, whereas the GR50 of the Fresno and Reedley plants ranged from 0.26 to 0.61 and 0.92 to 2.88 lb ae/ac, respectively. This study showed that the hairy fleabane plants from Reedley were much more tolerant of glyphosate than either of the other two biotypes and, based on the GR50, the level of resistance ranged from 3-to 10-fold greater.
Hairy or flax-leaved fleabane is a major weed infesting roadsides, ditch-banks, orchards, vineyards and fallow areas in California. This weed belongs to the Asteraceae family and is an annual or short-lived perennial native to South America (Noyes 2000). At the seedling stage, hairy fleabane (Conyza bonariensis) looks very similar to horse-weed (Conyza canadensis) and can often be found growing in the same locations. The most common methods used to control hairy fleabane in California include the use of pre-and postemergent herbicides, fall and/or spring cultivation, and hand-pulling. Generally, broad-spectrum postemergent herbicides, primarily glyphosate (Roundup), are used to control these weeds in orchards, vineyards and noncrop areas in California (Shrestha et al. 2007).
Glyphosate is considered the world's most important herbicide because it provides broad-spectrum weed control and has favorable environmental characteristics and low mammalian toxicity (Baylis 2000; Woodburn 2000). These characteristics, combined with its cost-effectiveness, have led to frequent applications of glyphosate in perennial cropping systems and noncrop areas in California (Shrestha et al. 2007). However, reliance on herbicides with the same mode of action for extended periods can contribute to weed shifts and the selection of biotypes with resistance to herbicides (Christoffers 1999; Holt 1992) (see page 86). As a result of this selection pressure, cases of glyphosate-resistant rigid rye-grass (Lolium rigidum) (Heap 2008; Simarmata et al. 2005) and horse-weed (Shrestha et al. 2007) have been reported in California orchards and non-crop areas, respectively.
Hairy fleabane plants survived glyphosate (Roundup Weathermax) applications on a reservoir bank in Fresno County. Over-reliance on herbicides with the same mode of action often leads to resistance.
In recent years, poor control of hairy fleabane with glyphosate has been observed in orchards, vineyards and noncrop areas of California. Since glyphosate-resistant horseweed has been documented in similar California locations (Shrestha et al. 2007), and glyphosate-resistant hairy fleabane has been reported in perennial crops in Spain (Urbano et al. 2007), we suspected glyphosate resistance in hairy fleabane after observing weeds that were not killed by labeled rates of glyphosate (escapes) at several sites. The objective of our study was to test for glyphosate resistance in hairy fleabane plants grown from seeds collected in perennial crop and noncrop locations around central California.
Seeds of hairy fleabane were collected in 2006 from a suspected glyphosate-resistant roadside population in Reedley (36° 29′15 N; 119°24′10 W). Seeds of suspected glyphosate-susceptible populations were collected from a vineyard in western Fresno (36°47′58 N; 119°57′16 W) and a non-crop area near Esparto (38°40′09 N; 122°01′11 W), where effective control is usually obtained with a labeled rate (22 fluid ounces per acre [fl oz/ac], i.e., 0.78 pounds acid equivalent per acre [lb ae/ac]) of glyphosate formulated as Roundup Weathermax.
Seeds were stored in a refrigerator in the laboratory at 40°F over the winter, planted in plastic germination trays in the laboratory in spring, and moved to a greenhouse following emergence. The day and night temperatures in the greenhouse were set at 75°F to 80°F and 60°F to 65°F, respectively. No supplemental lighting was used. When the seedlings developed two to three leaves, they were transplanted into plastic pots (6 inches deep, 4 inches wide) containing a commercial potting mix.
Experiments comparing the Esparto and Reedley populations and Fresno and Reedley populations were conducted separately and repeated. All the experiments were conducted between May and August. The experimental design was a completely randomized design with five replications. Each pot with a seedling from each location was an experimental unit. The plants were watered regularly and fertilized twice during the growing season with a commercial fertilizer (Miracle Gro).
The seedlings were treated with glyphosate (formulated as Roundup Weathermax) at rates of 0 (untreated), 0.19, 0.39, 0.78, 1.55, 3.1, 6.2 and 12.4 lb ae/ac. These rates correspond to 0, 5.5, 11, 22, 44, 88, 176 and 352 fl oz/ac of Roundup Weathermax. Treatments were applied with a moving-nozzle cabinet sprayer calibrated to deliver 17.7 gallons per acre through a single 8002 flat fan nozzle positioned 22 inches above the target plants.
Growth stage (leaf numbers) of the treated plants differed between experiments due to the duration of growth before treatment and conditions in the greenhouse. In the experiments comparing plants from Esparto and Reedley, the plants were treated with glyphosate at the 8-to-11-leaf stage and 18-to-22-leaf stage in the first and second runs of the experiment, respectively. In the experiments comparing the Fresno and Reedley plants, the plants were treated at the 12-to-15-leaf stage and 17-to-23-leaf stage in the first and second runs of the experiment, respectively.
Mortality and aboveground dry weight of the treated plants were recorded 2 weeks after glyphosate application. The plants were designated as “dead” when the aboveground plant parts showed no traces of green tissue (necrosis). All the plants, including the ones that died, were clipped at the surface of the soil, placed in separate paper bags, dried to constant weight in a forced-air oven at 140°F, and their dry weights were recorded. Some extra plants in each treatment were maintained up to a month after glyphosate application for visual assessments of damage and regrowth. Dry weights of the plants were expressed as percentage of control. Mortality data were subjected to analysis of variance using GLM procedures in SAS with an alpha level of 0.05. Dry weight data (% of control) were regressed against glyphosate dose, and a four-parameter logistic curve was fit using Sigma Plot with the following equation: γ=min + max−min 1 + 10 (GR50-X) where γ is plant dry weight (% of control), min is the minimum response limit, max is the maximum response limit, GR50 is the glyphosate dose required to reduce dry weight by 50% and x is the concentration of glyphosate.
Seedlings of, top, hairy fleabane and, bottom, horseweed. Populations of both weeds have now been shown to be resistant to glyphosate, a commonly used herbicide.
There were no interactions (P > 0.05) between the glyphosate rate and experimental run for plant mortality. However, in the biomass data, interactions (P < 0.05) occurred between glyphosate rate and experimental run. Therefore, data for plant mortality were combined for experimental rounds, whereas data for plant dry weight were analyzed separately for each run.
Plant mortality and dry weight
Plant mortality. In both experiments, all the seedlings from Fresno and Esparto were killed with a glyphosate rate of 1.55 lb ae/ac (figs. 1A, 1B). However, this was higher than the labeled rate (0.78 lb ae/ac) for hairy fleabane control. In both experiments, about 50% of the hairy fleabane plants from the suspected glyphosate-susceptible populations of Esparto and Fresno were able to survive the
In this study, glyphosate-resistant hairy fleabane recovered from injury 4 weeks after glyphosate was applied at 16 times the recommended rate.
Fig. 1. Average percent mortality (± SE) of hairy fleabane plants sprayed with different glyphosate rates from (A) Esparto and Reedley at the 8-to-11-leaf stage and 18-to-23-leaf stage and (B) Fresno and Reedley at the 12-to-15-leaf stage and 15-to-18-leaf stage.
These results showed that the plants from Reedley were glyphosate-resistant. Although the effect of growth stage on the level of resistance was not evaluated in this study, we suspect that the level of resistance could vary with growth stage. Interactions between glyphosate rate and plant growth stage have been reported in horseweed (Shrestha et al. 2007). The study also showed that although the hairy fleabane populations of Fresno and Esparto were generally susceptible to glyphosate, some plants escaped the labeled rate, and a glyphosate application at 1.55 lb ae/ac was required to kill these plants.
Plant shoot dry weight. Plant shoot dry weight expressed as percentage of untreated control varied between experiments (figs. 2 and 3). This may have been due to differences in the growth stages (leaf numbers) between the experiments. Urbano et al. (2007) have also reported that the glyphosate response of resistant and susceptible hairy fleabane plants was dependant on phenological stage. The GR50 of the glyphosate-susceptible and glyphosate-resistant plants increased with growth stage in their study. In our study, the GR50 of the Esparto and Reedley plants ranged from 0.28 to 0.30 and 0.92 to 2.88 lb ae/ac, respectively (fig. 2). Similarly, the GR50 of the Fresno and Reedley plants ranged from 0.26 to 0.61 and 1.62 to 1.79 lb ae/ac, respectively (fig. 3).
Based on these GR50 values, the hairy fleabane plants from Reedley showed at least a 3-fold level of resistance compared to the susceptible populations. However, the level of resistance ranged up to 10-fold depending on the growth stage of the plants at application. This result is consistent with the findings of Urbano et al. (2007), who also reported a 10-fold level of resistance. A phenomenon noticed in the plants that survived the highest rates of glyphosate was the appearance of new leaves from the center of the plant. Similar symptoms were also noticed in glyphosate-resistant horseweed.
Managing hairy fleabane
This study confirmed the presence of glyphosate-resistant hairy fleabane in noncrop areas of the Central Valley. It is very likely that glyphosate-resistant hairy fleabane is also present in cropped areas, particularly in orchards and vineyards of the Central Valley, because the windborne seeds of hairy fleabane can travel considerable distances with wind currents. Our results also indicated that the current labeled rate of glyphosate may not be sufficient to consistently control glyphosate-susceptible hairy fleabane populations at the early rosette stages. The chances of glyphosate escapes will be greater if applications are made after the plants have developed more than 10 leaves.
Because hairy fleabane can emerge in multiple flushes in late fall, late winter,
Fig. 2. Average shoot dry weights (± SE) of Esparto and Reedley hairy fleabane plants (expressed as percentage of untreated control) under different glyphosate rates in the (A) first (8-to-11-leaf stage) and (B) second (18-to-22-leaf stage) experimental run.